Browsing by Author "Tusiime, G."

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High Potassium, Calcium, And Nitrogen Application Reduce Susceptibility To Banana Xanthomonas Wilt Caused By Xanthomonas Campestris Pv. Musacearum
(Plant Disease, 2013-01-06) Atim, M.; Beed, F.; Tusiime, G.; Tripathi, L.; van Asten, P.
The effect of exogenous applications of potassium (K), calcium (Ca), and nitrogen (N) on the susceptibility of four banana cultivars to Ba- nana Xanthomonas wilt (BXW) was studied. Murashige and Skoog (MS) medium with normal concentrations of K at 783 mg/liter, Ca at 121 mg/liter, and N at 841 mg/liter was modified to contain various concentrations of K, Ca, and N. Each nutrient was varied singly, each with three replicate experiments. The concentrations were K at 78, 157, 391, 783, 1,565, and 3,913 mg/liter; Ca at 12, 24, 60, 121, 241, and 603 mg/liter; and N at 84, 168, 420, 841, and 1,682 mg/liter. Plant- lets were generated in vitro on normal MS medium and later exposed to the nutrient concentrations for a total of 8 weeks. Thereafter, they were artificially inoculated with Xanthomonas campestris pv. musa- cearum using an insulin syringe. In each nutrient, plantlets exposed to higher nutrient concentrations significantly (P < 0.0001) accumulated more nutrient in their tissues compared with those exposed to lesser nutrient concentrations. Wilt incidences were significantly reduced, and incubation periods (time from inoculation to appearance of first disease symptoms) increased, with increasing nutrient application. The study lays a background for in vivo studies aimed at management of BXW using nutrients, such as fertilizer application.
Role of Birds & Bats in Long Distance Transmission of Banana Bacterial Wilt in Uganda
(International Journal of Agriculture Innovations and Research, 2014-05-09) Buregyeya, H.; Kubiriba, J.; Tusiime, G.; Kityo, R.; Ssekiwoko, F.; Tushemerierwe., W. K.
Banana bacterial wilt caused by Xanthomonas campestri pv musacearum (Xcm) threatens the banana industry which contributes 22% of the GDP in Uganda. An important step in controlling this disease involves understanding how it is spread. The occurrence of isolated cases of disease in remote places in various districts far from the originally identified places suggest involvement of long distance vectors in the transmission. This study was initiated with the objective of establishing bats & birds’ vectors and transmission mechanisms of Xcm. An inventory of bats & birds species associated with banana inflorescence was carried out so as to investigate possible sources of inoculums in banana plants and determine bat and bird species that carried the bacterium on their bodies and thus possible vectors of the disease. The longest period the bacterium could stay viable on bats & birds mouth parts was determined. The purpose was to find out the possibility of involvement in long distance transmission of Xcm. The most birds visiting the male flowers are Eastern grey plantain eater, Double toothed barbet, Sunbird and village weaverbird. Bats that visited the male flower were Aidulon helvum, Epomophorus labiatus and Epomaps franquet. Bacterial cells have been isolated from these species and some of them were able to retain viable cells up to five days increasing the possibility of being involved in long distance transmission of Xcm.