Browsing by Author "Laban F. Turyagyenda"

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    Cassava Brown Streak Disease Prevalence in Smallholder Cassava Cropping Systems in Northern Uganda: The Case of Acholi Sub-region
    (East African Journal of Agriculture and Biotechnology, 2024-05-08) Alfred Kumakech; Allan Tekkara; Laban F. Turyagyenda
    Cassava brown streak disease (CBSD) is a devastating disease of cassava in Uganda. Limited information exists on the incidence and severity of CBSD in Northern Uganda. The prevalence of CBSD in the eight (8) districts in the Acholi sub-region is also unknown. Therefore, the current study intended to: a) determine the prevalence and severity of CBSD in the Acholi sub-region, and b) identify the drivers of CBSD epidemics in smallholder cassava cropping systems in Acholi. An assessment of 120 cassava fields was conducted in the 2018b season. CBSD field incidence was highest in Nwoya District (51.3%) and lowest in Kitgum (6.4%), with severity ranging from 2.1 to 3.4 in the sub-region. CBSD prevalence was also highest in Nwoya (76.2%) and lowest in Kitgum and Pader Districts (30.8%). The use of CBSD-susceptible varieties (TME 14, TME 204, and NASE 12) was identified as the main driver of the epidemic. The result of the current study highlights the need for a wide-scale CBSD awareness creation and community-based cassava seed multiplication and distribution system in the Acholi sub-region to promote the up-take of CBSD-tolerant cassava varieties.
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    Pseudocercospora fijiensismycelia-based infection system enhances investigational efficacy of P. fijiensis-banana pathosystem
    (Brazilian Journal of Science, 2024-02-01) Kumakech Alfred; Laban F. Turyagyenda; Richard Edema; Patrick Okori
    Screening procedures for black Sigatoka have limitations. Thus, there is need for alternative screening procedure. A robust controlled-environment methodology for testing reaction of banana genotypes to Pseudocercospora fijiensis is, thus, still required. The objective of this study was, therefore, to assess the effect of P. fijiensis fragmented mycelia-based inoculum on black Sigatoka development in banana under screen house conditions with the view of developing a procedure for early assessment of resistance. Black Sigatoka severity increased significantly (P < 0.05) over time in all genotypes apart from Kayinja. Significant differences (P < 0.05) in severity were also recorded among the genotypes at 3, 4, 5 and 6 weeks after inoculation. All east African highland banana and plantain genotypes tested exhibited susceptible reaction, characterized by rapid progression of symptoms to necrotic lesions. Kayinja and M9 hybrids exhibited resistant reactions, characterized by small necrotic specks and chlorotic or brown blotches, respectively. The fragmented mycelia-based infection system classified the banana genotypes into resistant and susceptible clones, making it a reliable and efficient infection technique to assess black Sigatoka disease damage. The infection system is recommended for early screening for black Sigatoka resistance.